The investigation was undertaken to study some blood biochemical parameters and cortisol levels during different stages in different lactations in Toggenberg goats. These included control group, consisting of dry goats and group I, II, III and IV containing goats in 1st, 2nd, 3rd and 4th lactation period. The total protein concentration showed an increasing trend from early to late lactation stages. There was an increasing levels of albumin in group I, II and IV and globulin in group II and III. Lowest total protein concentration was observed in third lactation (group III). Albumin and globulin ratio was higher in early lactation in group III (third lactation); whereas, in group II and IV, highest ratio was observed during mid lactation. Glucose concentration showed a defi nite increasing trend from early to late lactation stages in different lactations. Signifi cantly lower (P<0.05) glucose levels were found in early as well as mid lactations as compared to late stage in all the four groups. Increasing trend of urea concentration was observed from early to late stage of lactation in all the lactating groups. The Total protein, globulin, glucose and urea levels were found higher in lactating goats as compared to dry animals. The cortisol level showed a decreasing trend from early to late stage of lactation, Again, the cortisol levels of lactating goats were signifi cantly higher (P<0.05) than that of dry goats. Among different stages of lactation, early and mid stages are more stressful when compared to late stage.